Journal of Operative Dentistry & Endodontics

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VOLUME 2 , ISSUE 1 ( January-June, 2017 ) > List of Articles

ORIGINAL ARTICLE

Caries-preventive Efficacy of Resin Infiltrant, Casein Phosphopeptide-amorphous Calcium Phosphate, and Nanohydroxyapatite using Confocal Scanning Electron Microscope: An in vitro Study

A Arvindkumar, Uma Maheswari, C Kingston, R Gnanaseelan, R Jonathan Emil Sam

Citation Information : Arvindkumar A, Maheswari U, Kingston C, Gnanaseelan R, Jonathan Emil Sam R. Caries-preventive Efficacy of Resin Infiltrant, Casein Phosphopeptide-amorphous Calcium Phosphate, and Nanohydroxyapatite using Confocal Scanning Electron Microscope: An in vitro Study. J Oper Dent Endod 2017; 2 (1):6-10.

DOI: 10.5005/jp-journals-10047-0025

License: CC BY 3.0

Published Online: 01-06-2017

Copyright Statement:  Copyright © 2017; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Introduction

White spot lesion is the early sign of demineralization occurring under intact enamel which may lead to the development of caries.

Aim

The aim of this study is to compare and evaluate the caries-preventive efficacy of a resin infiltrant, casein phosphopeptideamorphous calcium phosphate (CPP-ACP), and nanohydroxyapatite (nano-HA) on white spot enamel lesions.

Settings and designs

A total of 40 freshly extracted human maxillary incisors were used in this study. Enamel samples (2 mm thickness) were prepared and sample preparation windows were created (dimension of 5 × 5 mm) using adhesive tape, and the sample was made completely resistant to acid attack by coating nail varnish.

Materials and methods

The samples were divided into four groups, which are resin infiltrant, CPP-ACP, nano-HA, and control of 10 enamel samples in each group. The samples were evaluated using confocal laser scanning microscopy before and after the application of resin infiltrant and remineralizing agents.

Statistical analysis

Statistical analysis was done using analysis of variance and post hoc Bonferroni test was used for comparing intragroups and Tukey test for comparing intergroups.

Results

The mean value after demineralization is 245, 246, 250, and 247 μm for Groups I to IV. After remineralization, group I > group II > group III > group IV. After acid challenge for a period of 14 days, group I > group II > group III > group IV.

Conclusion

The resin infiltrant showed higher caries inhibition potential and superior acid resistance than CPP-ACP and nano-HA.

Clinical significance

The inhibition of caries progression by resin infiltration technique should be considered as an alternative approach to the more invasive therapies and warrants a place in the range of minimally invasive dentistry techniques.

How to cite this article

Arvindkumar A, Maheswari U, Kingston C, Gnanaseelan R, Jonathan Emil Sam R. Caries-preventive Efficacy of Resin Infiltrant, Casein Phosphopeptide-amorphous Calcium Phosphate, and Nanohydroxyapatite using Confocal Scanning Electron Microscope: An in vitro Study. J Oper Dent Endod 2017;2(1):6-10.


  1. Caries lesions after orthodontic treatment followed by quantitative light induced fluorescence: a 2 year follow up. Eur J Oral Sci 2007 Jun;29(3):294-298.
  2. ; Fejerskov O.; Cury JA.; Clarkson J. Fluorides in caries control. In: Fejerskov O, Kidd E, editors. Dental caries. The disease and its clinical management. Oxford: Blackwell Munksgaard; 2008. p. 287-327.
  3. Light-induced fluorescence studies on dehydration of incipient enamel lesions. Caries Res 2002 Jan-Feb;36(1):25-30.
  4. Minimally invasive treatment of white spot enamel lesions. Quintessence Int 2007 Sep;38(8):633-636.
  5. Minimal intervention dentistry: part 6. Caries inhibition by resin infiltration. Br Dent J 2013 Jan;214(2):53-59.
  6. Enamel subsurface lesion remineralization with case in phosphopeptide stabilized solutions of calcium, phosphate and fluoride. Caries Res 2008 Jan;42(2):88-97.
  7. Anticariogenic complexes of amorphous calcium phosphate stabilized by casein phosphopeptides: a review. Spec Care Dentist 1998 Jan-Feb;18(1):8-16.
  8. Remineralization potential of nano-hydroxyapatite on initial enamel lesions: an in vitro study. Caries Res 2011 Sep;45(5):460-468.
  9. Using hydroxyapatite nano particles and decreased crystallinity to promote osteoblast adhesion similar to functionalizing with RGD. Biomaterials 2006 May;27(14):2798-2805.
  10. Resin-dentin interface studied by SEM and CLSM. Microsc Anal 1996;42(1):15-16.
  11. Fluoride taken up by plaque, by the underlying enamel and by clean enamel from three fluoride compounds in vitro. Caries Res 1982;16(2):156-161.
  12. Lesion formation and lesion remineralization in enamel under constant composition conditions. A new technique with applications. Caries Res 1985;19(6):490-496.
  13. Dental caries process. Dent Clin North Am 1999 Oct;43(4):635-636.
  14. Remineralization phenomena. Caries Res 1977;11(Suppl 1):59-84.
  15. Remineralization of softened bovine enamel following treatment of overlying plaque with a mineral-enriching solution. J Dent Res 1985 Mar;64(3):416-421.
  16. Structure of carious enamel, including the early lesion. Oral Sci Rev 1973;3:100-160.
  17. Improved resin infiltration of natural caries lesions. J Dent Res 2008 Dec;87(12):1112-1116.
  18. Inhibition of lesion progression by the penetration of resins in vitro: influence of the application procedure. Oper Dent 2006 May-Jun;31(3):338-345.
  19. Penetration coefficients of commercially available and experimental composites intended to infiltrate enamel carious lesions. Dent Mater 2007 Jun;23(6):742-748.
  20. Resin infiltration of artificial enamel caries lesions with experimental light curing resins. Dent Mater J 2007 Jul;26(4):582-588.
  21. Resin infiltration of natural caries lesions. J Dent Res 2007 Jul;86(7):662-666.
  22. Influence of the application time on the penetration of different dental adhesives and a fissure sealant into artificial subsurface lesions in bovine enamel. Dent Mater 2006 Jan;22(1):22-28.
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