Aim: To evaluate the antibacterial efficacy of dopamine incorporated total-etch adhesive against Streptococcus mutans (S. mutans).
Materials and methods: S. mutans were cultured and inoculated in Müeller–Hinton agar plates. Round wells of around 6 mm diameter were created in the center of the agar plates. The experimental groups consisted of agents as follows: group I (DOPA 3%), group II (DOPA 3% + Bonding agent), group III (Bonding agent) and group IV (no material). For each group, 12 plates were used to evaluate the zone of inhibition using agar well diffusion method. All the experimental agents were added to respective wells in agar plates and incubated at 37° C for 24 hours. The diameter of a zone of inhibition around each well was recorded. Results were tabulated and analyzed statistically using the Kruskal–Wallis test. and Mann-Whitney U-test.
Results: Based on the mean diameters, group II showed the maximum zone of inhibition, and it exhibited statistically higher antibacterial activity than group III and group I (p < 0.01).
Conclusion: Within the limitations of this study, dopamine incorporated total-etch adhesive system exhibited significantly higher antibacterial activity than conventional adhesive system against S. mutans.
Clinical significance: Dopamine can be a promising antibacterial additive to dental adhesive systems to improve both biological seal and bond strength at the resin-dentin interface.
Chrysanthakopoulos NA. Reasons for Placement and Replacement of Resin-based Composite Restorations in Greece. J Dent Res Dent Clin Dent Prospects. 2011;5(3):87-93.
Soares CJ, Faria ES, Rodrigues MDP, Vilela ABF, Pfeifer CS, Tanbiio DM et al. Polymerization shrinkage stress of composite resins and resin cements What do we need to know? Braz. Oral Res. 2017;31(suppl):e62:49-63.
Liu Y, Tjäderhane L, Breschi L, Mazzoni A, Li N, Mao J, Tay FR. Limitations in Bonding to Dentin and Experimental Strategies to Prevent Bond Degradation. J Dent Res. 2011;90:953-968.
Amin S, Shetty HK, Varma RK, Amin V, Nair PMS. Comparative evaluation of antibacterial activity of total-etch and self-etch adhesive systems: An ex vitro study. J Conserv Dent. 2014;17(3):266-270.
Camps J, Pashley DH. Buffering action of human dentin in vitro. J Adhes Dent 2000;2(1):39-50.
Bapna MS, Murphy R, Mukherjee S. Inhibition of bacterial colonization by antimicrobial agents incorporated into dental resins. J Oral Rehab. 1988;15:405-411.
Cheng L, Zhang K, Melo MA, Weir MD, Zhou X, Xu HH. Antibiofilm dentin primer with quaternary ammonium and silver nanoparticles. J Dent Res. 2012;91:598-604.
Imazato S, Kuramoto A, Takahashi Y, Ebisu S, Peters MC. In vitro antibacterial effects of the dentin primer of Clearfil Protect Bond. Dent Mater. 2006;22:527-532.
Cheng L, Weir MD, Xu HH, Kraigsley AM, Lin NJ, Lin GS, Zhou X. Antibacterial and physical properties of calciumphosphate and calcium-fluoride nanocomposites with chlorhexidine. Dent Mater. 2012;28:573-583.
Tziafas D, Kolinioto-K E, Tziafa C, Papadimitriou S. Effects of a new antibacterial adhesive on the repair capacity of the pulpdentine complex in infected teeth. Int Endod J. 2007;40(1): 58-66.
Lobo MM, Goncalves RB, Pimenta LA, Bedran-Russo AK, Pereira PN. In vitro evaluation of caries inhibition promoted by self-etching adhesive systems containing antibacterial agents. J Biomed Mater Res B Appl Biomater. 2005;75(1):122-127.
Rolland SL, McCabe JF, Robinson C, Walls AW. In vitro biofilm formation on the surface of resin-based dentine adhesives. Eur J Oral Sci. 2006;114(3):243-249.
Hahnel S, Leyer A, Rosentritt M, Handel G, Burgers R. Surface properties and in vitro Streptococcus mutans adhesion to selfetching adhesives. J Adhes Dent. 2009;11(4):263-269.
Ding YH, Floren M, Tan W. Mussel-inspired polydopamine for bio-surface functionalization. Biosurface and Biotribology. 2016;2(4):121-136.
Jo SH, Sohn JS. Biomimetic Adhesive Materials Containing Cyanoacryl Group for Medical Application. Molecules. 2014;19(10): 1679-1693.
Ho CC, Ding SJ. Structure, properties, and applications of mussel-inspired polydopamine. J Biomed Nanotechnol. 2014;10(10):3063-3084.
Sang BL, Carlos GC, Kwang MK, Kyoung NK, Kenichi K. Catechol-Functionalized Synthetic Polymer as a Dental Adhesive to Contaminated Dentin Surface for a Composite Restoration. Biomacromolecules. 2015;16(8):2265-2275.
Mei ML, Li QL, Chu CH. Inhibition of cariogenic plaque formation on root surface with polydopamine-induced-polyethylene glycol coating. Materials (Basel). 2016;9(6):414-426.
Harper DS, Loesche WJ. Growth and acid tolerance of human dental plaque bacteria. Arch Oral Biol 1984;;29(10):843-848.
Kuramitsu HK. Virulence factors of mutans streptococci role of molecular genetics. Crit Rev Oral Biol Med. 1993;4(2):159-176.
Vanajasan PP, Dhakshinamoorthy M, Rao CS. Factors affecting the bond strength of self-etch adhesives: A meta-analysis of literature. J Cons Dent 2011;14(1):62-67.
Dalsin JL, Hu BH, Lee BP, Messersmith PB. Mussel adhesive protein mimetic polymers for the preparation of nonfouling surfaces. J Am Chem Soc 2003;125(14):4253-4258.
Cristiane D, Thais CN, Denise MPS. Josimeri H. Effect of light-activation on the antibacterial activity of dentin bonding agents 2009;8(4):175-180.
Atac AS, Cehreli ZC, Sener B. Antibacterial activity of fifth-generation dentin bonding systems. J Endod. 2001;27(12):730-733.
Baseren M, Yazici AR, Ozalp M, Dayangac B. Antibacterial activity of different generation dentin-bonding system. Quintessence Int. 2005;36(5):339-344.
Iqbal Z, Lai EPC, Avis TJ. Antimicrobial effect of polydopamine coating on Escherichia coli. J Mater Chem 2012;22(40): 21608-21612
Maji S, Maji HS, Chakraborthy P, Dastidar S. potential of dopamine hydrochloride as an novel antimicrobial agent. International journal of biomedical and pharmaceutical sciences. 2010;4(2):70-75.
Ji Y, Ji T, Liang K, Zhu L. Mussel-inspired soft-tissue adhesive based on poly(diol citrate) with catechol functionality. J Mater Sci Mater Med 2016;27(2):30.
Yang FK, Zhao B. Adhesion properties of self-polymerized dopamine thin film, Surface Science 2011;3(1):115-122.