Journal of Operative Dentistry & Endodontics

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VOLUME 3 , ISSUE 2 ( December, 2018 ) > List of Articles


Antibacterial Activity of Dopamine Incorporated Total Etch Adhesive System

H Sandeep Adimulapu, Madhubala M Madhana, Mahalaxmi Sekar

Keywords : Antibacterial, Dopamine, S. mutans, Total etch adhesive

Citation Information : Adimulapu HS, Madhana MM, Sekar M. Antibacterial Activity of Dopamine Incorporated Total Etch Adhesive System. J Oper Dent Endod 2018; 3 (2):53-56.

DOI: 10.5005/jp-journals-10047-0057

License: CC BY-NC 3.0

Published Online: 01-01-2019

Copyright Statement:  Copyright © 2018; The Author(s).


Aim: To evaluate the antibacterial efficacy of dopamine incorporated total-etch adhesive against Streptococcus mutans (S. mutans). Materials and methods: S. mutans were cultured and inoculated in Müeller–Hinton agar plates. Round wells of around 6 mm diameter were created in the center of the agar plates. The experimental groups consisted of agents as follows: group I (DOPA 3%), group II (DOPA 3% + Bonding agent), group III (Bonding agent) and group IV (no material). For each group, 12 plates were used to evaluate the zone of inhibition using agar well diffusion method. All the experimental agents were added to respective wells in agar plates and incubated at 37° C for 24 hours. The diameter of a zone of inhibition around each well was recorded. Results were tabulated and analyzed statistically using the Kruskal–Wallis test. and Mann-Whitney U-test. Results: Based on the mean diameters, group II showed the maximum zone of inhibition, and it exhibited statistically higher antibacterial activity than group III and group I (p < 0.01). Conclusion: Within the limitations of this study, dopamine incorporated total-etch adhesive system exhibited significantly higher antibacterial activity than conventional adhesive system against S. mutans. Clinical significance: Dopamine can be a promising antibacterial additive to dental adhesive systems to improve both biological seal and bond strength at the resin-dentin interface.

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