Journal of Operative Dentistry & Endodontics

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VOLUME 8 , ISSUE 2 ( July-December, 2023 ) > List of Articles

Original Article

Evaluation of Various Concentrations of Punica granatum Extract Gel's Potency in Teeth Whitening: An In Vitro Study

S Advaitha Narayan, Selvendran K Elangovan, K Madhuram, Ashok Leburu, Naveen Kumar, N Praveen

Keywords : Bleaching agent, CIE color lab, Natural remedy, Surface roughness, Teeth bleaching, White pomegranate

Citation Information : Narayan SA, Elangovan SK, Madhuram K, Leburu A, Kumar N, Praveen N. Evaluation of Various Concentrations of Punica granatum Extract Gel's Potency in Teeth Whitening: An In Vitro Study. J Oper Dent Endod 2023; 8 (2):25-31.

DOI: 10.5005/jp-journals-10047-0133

License: CC BY-NC 4.0

Published Online: 27-09-2024

Copyright Statement:  Copyright © 2023; The Author(s).


Abstract

Teeth whitening is a conservative procedure utilized to overcome tooth discoloration. Malic acid and ellagic acid obtained from white pomegranate can be used to whiten teeth. Aim: The purpose of this research was to evaluate the efficacy of white pomegranate extract gel as a bleaching agent against tooth discoloration and its effects on the enamel surface. Materials and methods: Forty-eight mandibular incisors were utilized as samples. All teeth were submerged for 12 days in the coffee solution. Utilizing spectrophotometry, tooth discoloration was measured. After being soaked in a coffee solution and then in a white pomegranate extract gel, the item was dried. Group I: Ten teeth soaked in white pomegranate extract gel with a concentration of 70%. Group II: Ten teeth soaked in white pomegranate extract gel with a concentration of 80%. Group III: Ten teeth soaked in white pomegranate extract gel with a concentration of 90%. Group IV: Around 10% of carbamide peroxide gel as a positive control for 10 teeth. Each specimen was immersed for 4 hours per day for 7 days. Using a spectrophotometer, tooth discoloration was assessed following immersion in white pomegranate gel extract and carbamide peroxide gel, and with a stylus profilometer, the surface roughness of the enamel was assessed. Results: The Delta E value of 90% white pomegranate extract gel was higher than the positive control. The average surface roughness value of white pomegranate extract gel increased with an increase in its concentration; hence, 70% of white pomegranate extract gel had the least enamel surface roughness. Conclusion: Based on the results, group III (90% white pomegranate extract gel) was determined to be the most effective as a teeth-whitening agent, and group I (70% white pomegranate extract gel) showed the least surface roughness. White pomegranate extract gel has an influence on tooth discoloration and teeth whitening attempts.


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  1. Ercisli S, Gadze J, Agar G, et al. Genetic relationships among wild pomegranate (Punica granatum) genotypes from Coruh Valley in Turkey. Genet Mol Res 2011;10:459–464. DOI: 10.4238/vol10-1gmr1155.
  2. Newman RA, Lansky EP, Block ML. Pomegranate: The Most Medicinal Fruit. Laguna Beach, California: Basic Health Publications; 2007. A Wealth of Phytochemicals; p. 120.
  3. Gil MI, Garcia-Viguera C, Artes F, et al. Changes in pomegranate juice pigmentation during ripening. J Sci Food Agric 1995;68:77–81. DOI: 10.1002/jsfa.2740680113.
  4. Ben-Simhon Z, Judeinstein S, Trainin T, et al. A “White” anthocyanin-less pomegranate (Punica granatum L.) caused by an insertion in the coding region of the leucoanthocyanidin dioxygenase (LDOX; ANS) gene. PLoS ONE 2015;10(11):e0142777. DOI: 10.1371/journal.pone.0142777.
  5. Asmawati MA. Pemanfaatan Buah Strawberry sebagai Bahan Pemutih Gigi. Makasar Dent J 2016;5(2):40–43. DOI: 10.35856/mdj. v5i2.96.
  6. Margaretha J, Rianti D, Meizarini A. Perubahan Warna Enamel Gigi Setelah Aplikasi Pasta Buah Stroberi dan Gel Karbamid Perosida 10% (Effect of strawberry paste and carbamide peroxide gel 10% towards the brightness enamel tooth). Mater Dent J 2009; 1(1):1620. DOI: 10.37275/cmej.v5i1.440.
  7. Meireles SS, Heckmann SS, Santos IS, et al. A double blind randomized clinical trial of at-home tooth bleaching using two carbamide peroxide concentrations: 6-month follow-up. J Dent 2008;36(11): 878–884. DOI: 10.1016/j.jdent.2008.07.002.
  8. Khan MSA, Ahmad I, Chattopadhyay D. New Look to Phytomedicine. Academic Press; 2019, pp. 657–685. DOI: 10.1016/B978-0-12-814619-4.00 040-9.
  9. Radhakrishnan R, Nesamani RM, Sanjeev K, et al. Effect of bleaching with strawberry extract and hydrogen peroxide on colour change, surface morphology and micro-shear bond strength of resin composite to enamel surface. J Clin Diagn Res 2021;15(3):ZC01–ZC06. DOI: 10.7860/JCDR/2021/47070.14601.
  10. Mirsane S, Mirsane S. Benefits of ellagic acid from grapes and pomegranates against colorectal cancer. Caspian J Intern Med 2017;8:226–227. DOI: 10.22088/cjim.8.3.226.
  11. Dewiyani S, Widyastuti R, Rosanti N. Potency of acid content in local and imported strawberry fruit extract (Fragaria X Ananassa) as natural ingredients for teeth whitening. J Dental Sci 2023;8(3):000377. DOI: 10.23880/oajds-16000377.
  12. Seow WK, Thong KM. Erosive effects of common beverages on extracted premolar teeth. Aust Dent J 2005;50(3):173–178; quiz 211. DOI: 10.1111/j.1834-7819.2005.tb00357.x.
  13. Basak JK, Madhavi BGK, Paudel B, et al. Prediction of total soluble solids and pH of strawberry fruits using RGB, HSV and HSL colour spaces and machine learning models. Foods 2022;11(14):2086. DOI: 10.3390/foods11142086.
  14. Asmawati A, Rieuwpassa IE. Comparison of enamel hardness after the application of dental bleaching agents strawberry gel and 10% carbamide peroxide. J Dentomaxillofacial Sci 2018;3(1):17–19. DOI: 10.15562/jdmfs.v3i1.626.
  15. Abdelaleem NA, Nassar AA, Alhindi AK, et al. Dental staining associated with various types of coffee. J Res Med Dent Sci 2022;10(9): 254–259. pISSN No. 2347-2545.
  16. Muga IFR, Meizarini A, Nirwana I, et al. The use of white pomegranate extract as a bleaching material for hardness, roughness, and calcium levels in the enamel tooth. World J Adv Res Rev 2023;18(02):700–704. DOI: 10.30574/wjarr.2023.18.2.0855.
  17. Majeed A, Farooq I, Grobler SR, et al. Tooth-bleaching: A review of the efficacy and adverse effects of various tooth whitening products. J Coll Physicians Surg Pak 2015;25(12):891–896. PMID: 26691365.
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