Histological Evaluation of Pulpal Response to Direct Pulp Capping with Biodentine and Mineral Trioxide Aggregate: An In Vivo Study
Hema J Christa, Shakthi Priya Sivaprakasam
Dentin bridge, Direct pulp capping, Inflammation, Mineral trioxide aggregate, Orthodontic extraction,Biodentine
Citation Information :
Christa HJ, Sivaprakasam SP. Histological Evaluation of Pulpal Response to Direct Pulp Capping with Biodentine and Mineral Trioxide Aggregate: An In Vivo Study. J Oper Dent Endod 2019; 4 (2):57-61.
Aim: The aim of the present study is to compare the pulpal response to direct pulp capping with the mineral trioxide aggregate (MTA) and biodentine by light microscopic histological evaluation.
Materials and methods: A total of 45 premolars scheduled for orthodontic extraction were selected and subjected to the direct pulp capping procedure. Class I cavities were prepared and MTA or biodentine was placed over the exposed pulp, followed by a composite restoration. After the experimental periods of 7, 30, and 90 days, the teeth were extracted and histological processing was carried out. The test materials were evaluated histologically for the degree of inflammation, dentin bridge formation, and thickness of dentin bridge at all the three different observation periods, tabulated and statistically analyzed using the Chi-square test.
Results: At 7 days\' observation, all the samples in groups I and II showed a mild inflammatory response. At 1 month, a thin or partial dentin bridge was evident in all the samples in both groups. At 3 months, all the samples in both groups showed evidence of complete dentin bridge formation. There was no statistically significant difference between the MTA and biodentine groups.
Conclusion: Biodentine was as effective as MTA in inducing a pulpal reaction with minimal inflammation and in dentin bridge formation.
Glossary of Endodontic terms. 7th edn, Chicago (IL): American Association of Endodontists; 2003.
Wataha JC. Biocompatibility of dental materials. In: Craig RG, Powers JM. Restorative dental materials. St. Louis: Mosby; 2002. 125–162.
Cox CF, Subay RK, Ostro E, et al. Tunnel defects in dentin bridges: their formation following direct pulp capping. Oper Dent 1996;21(1):4–11.
Dammaschke T, Wolff P, Sagheri D, et al. Mineral trioxide aggregate for direct pulp capping: a histologic comparison with calcium hydroxide in rat molars. Quintessence Int 2010;41(2):20–30.
Zarrabi MH, Javidi M, Jafarian AH, et al. Histologic assessment of human pulp response to capping with mineral trioxide aggregate and a novel endodontic cement. J Endod 2010;36(11):1778–1781. DOI: 10.1016/j.joen.2010.08.024.
Mente J, Geletneky B, Ohle M. Mineral trioxide aggregate or calcium hydroxide direct pulp capping: an analysis of the clinical treatment outcome. J Endod 2010;36(5):806–813. DOI: 10.1016/j.joen.2010.02.024.
Aeinehchi M, Eslami B, Ghanbariha M, et al. Mineral trioxide aggregate (MTA) and calcium hydroxide as pulp_capping agents in human teeth: a preliminary report. Int Endod J 2003;36(3):225–231. DOI: 10.1046/j.1365-2591.2003.00652.x.
Nair PN, Duncan HF, Pitt Ford TR, et al. Histological, ultrastructural and quantitative investigations on the response of healthy human pulps to experimental capping with mineral trioxide aggregate: a randomized controlled trial. Int Endod J 2008;41(2):128–150.
Hegde S, Sowmya B, Mathew S, et al. Clinical evaluation of mineral trioxide aggregate and biodentine as direct pulp capping agents in carious teeth. J Conserv Dent 2017;20(2):91–95. DOI: 10.4103/0972-0707.212243.
Masaki T, Yasuhisa T, Atsushi O, et al. Timing for composite resin placement on mineral trioxide aggregate. J Endod 2013;39(9): 116–1170.
Yasuda Y, Ogawa M, Arakawa T, et al. The effect of mineral trioxide aggregate on the mineralization ability of rat dental pulp cells: an in Vitro study. J Endod 2008;34(9):1057–1060. DOI: 10.1016/j.joen.2008.06.007.
Seo M, Hwang K, Lee J. The effect of mineral trioxide aggregate on Odontogenic differentiation in dental pulp stem cells. J Endod 2013;39(2):242–248. DOI: 10.1016/j.joen.2012.11.004.
Zhou HM, Shen Y, Wang ZJ, et al. In vitro cytotoxicity evaluation of a novel root repair material. J Endod 2013;39:478–483. DOI: 10.1016/j.joen.2012.11.026.
Laurent P, Camps J, De Méo M, et al. Induction of specific cell responses to a Ca3SiO5-based posterior restorative material. Dent Mater 2008;24(11):1486–1494. DOI: 10.1016/j.dental.2008.02.020.
Tran XV, Gorin C, Willig C, et al. Effect of a calcium-silicate-based restorative cement on pulp repair. J Dent Res 2012;91:1166–1171. DOI: 10.1177/0022034512460833.
Peng W, Liu W, Zhai W, et al. Effect of tricalcium silicate on the proliferation and odontogenic differentiation of human dental pulp cells. J Endod 2011;37(9):1240–1246. DOI: 10.1016/j.joen.2011.05.035.
Lipski M, Nowicka A, Kot K, et al. Factors affecting the outcomes of direct pulp capping using biodentine. Clin Oral Investig 2018;22(5):2021–2029. DOI: 10.1007/s00784-017-2296-7.
Jalan AL, Warhadpande MM, Dakshindas DM. A comparison of human dental pulp response to calcium hydroxide and biodentine as direct pulp-capping agents. J Conserv Dent 2017;20(2):129–133. DOI: 10.4103/0972-0707.212247.