Journal of Operative Dentistry & Endodontics

Register      Login

VOLUME 2 , ISSUE 2 ( July-December, 2017 ) > List of Articles

RESEARCH ARTICLE

Effects of Different Final Irrigating Solutions on Depth of Sealer Penetration into Dentinal Tubules: An in vitro Confocal Laser Microscopic Study

Madhavi A Shetty

Citation Information : Shetty MA. Effects of Different Final Irrigating Solutions on Depth of Sealer Penetration into Dentinal Tubules: An in vitro Confocal Laser Microscopic Study. J Oper Dent Endod 2017; 2 (2):69-73.

DOI: 10.5005/jp-journals-10047-0040

Published Online: 01-12-2017

Copyright Statement:  Copyright © 2017; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim

To evaluate the maximum depth of sealer penetration into dentinal tubules at the coronal, middle, and apical third of root canals, following the use of different final irrigation solutions.

Materials and methods

Eighty freshly extracted mandibular premolars were selected for the study. After decoronation, instrumentation of samples was done using Protaper universal rotary file system till F4. During shaping and cleaning of root canal, 5.25% concentration of sodium hypochlorite (NaOCl) was used for irrigation. The samples were then randomly divided into four groups (n = 20) depending on the final irrigating solution used. Group I: 17% aqueous ethylenediaminetetraacetic acid (MA) (aq. EDTA); group II: 10% citric acid (CA); group III: 5% maleic acid; group IV: 5.25% conc. NaOCl. Passive ultrasonic irrigation (PUI) was used to activate each final irrigating solution. The samples were obturated using warm lateral condensation technique with guttapercha coated with AH plus sealer mixed with rhodamine B dye. Confocal laser scanning microscopy (CLSM) examined the sealer penetration depth.

Results

The Kruskal-Wallis analysis results showed that there was a significant difference in the maximum depth of sealer penetration among all groups in all sections (p < 0.05). The coronal sections of the root canals in each group showed a significantly higher depth of sealer penetration than did the apical and middle sections (p < 0.05).

Conclusion

A combination of 17% aq. EDTA and PUI used for final irrigation proved to be more effective for sealer penetration into the dentinal tubules.

Clinical significance

Final irrigation protocol followed is of paramount importance in achieving complete disinfection within the root canal, and also the penetration of sealer serves as an indicator of the extent to which the smear layer was removed.

How to cite this article

Shetty MA, Hegde V. Effects of Different Final Irrigating Solutions on Depth of Sealer Penetration into Dentinal Tubules: An in vitro Confocal Laser Microscopic Study. J Oper Dent Endod 2017;2(2):69-73.


PDF Share
  1. Evaluation and comparison of biological cleaning efficacy of two endofiles and irrigants as judged by microbial quantification in primary teeth-an in vivo study. Int J Clin Pediatr Dent 2009 Sep-Dec;2(3):15-22.
  2. Effect of different final irrigation solutions on dentinal tubule penetration depth and percentage of root canal sealer. J Endod 2012 Jun;38(6):860-863.
  3. Effect of three different time periods of irrigation with EDTA-T, EDTA and citric acid on smear layer removal. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004 Oct;98(4):499-503.
  4. The smear layer in endodontics-a review. Int Endod J 2010 Jan;43(1):2-15.
  5. Recent concepts on root canal chelation. ENDO (Lond Engl) 2011;5(3):175-194.
  6. Chelating agents in root canal treatment: Mode of action and indications for their use. Int Endod J 2003 Dec;36(12):810-830.
  7. Chitosan: a new solution for removal of smear layer after root canal instrumentation. Int Endod J 2013 Apr;46(4):332-338.
  8. Review of contemporary irrigant agitation techniques and devices. J Endod 2009 Jun;35(6):791-804.
  9. Interfacial adaptation of adhesive materials to root canal dentin. J Endod 2007 Mar;33(3):259-263.
  10. A preliminary study of the percentage of sealer penetration in roots obturated with the Thermafil and RealSeal-1 obturation techniques in mesial root canals of mandibular molars. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009 Dec;108(6):961-968.
  11. ; Yee, FS. Canal debridement and disinfection. In: Cohen S.; Burns RC., editors. Pathways of the pulp. 3rd ed. The St. Louis: CV Mosby Company, 1984. p. 175.
  12. Root canal anatomy of the human permanent teeth. Oral Surg Oral Med Oral Pathol 1984 Nov;58(5):589-599.
  13. Medicaments: aids to success in endodontics. Part 1. A review of the literature. Aust Dent J 1990 Oct;35(5):438-448.
  14. Effect of EDTA with and without surfactants or ultrasonics on removal of smear layer. J Endod 2007 Apr;33(4):472-475.
  15. The effect of EDTA with and without ultrasonics on removal of the smear layer. J Endod 2009 Mar;35(3):393-396.
  16. Use of fluorescent compounds in assessing bonded resin-based restorations: a literature review. J Dent 2006 Oct;34(9):623-634.
  17. Comparison of the apple vinegar with otherchelating solutions on smear layer and calcium ions removal from the root canal. Indian J Dent Res 2014 May-Jun;25(3):370-374.
  18. Scanning electron microscopic study of the effects of various irrigants and combinations on the walls of prepared root canals. J Endodon 1982;11:487-492.
  19. A scanning electron microscopic comparison of a high volume final flush with several irrigating solutions. Part 3. J Endodon 1983 Apr;9(4):137-142.
  20. A contemporary overview of endodontic irrigants-a review. J Dent App 2014;1(6): 105-115.
  21. Effect of EDTA and citric acid solutions on the microhardness and the roughness of human root canal dentin. J Endod 2005 Feb;31(2):107-110.
  22. Effect of chelating solutions on the microhardness of root canal lumen dentin. J Endod 2011 Mar;37(3):358-362.
  23. The effectiveness of ultrasonic irrigation to remove artificially placed dentine debris from different-sized simulated plastic root canals. Int Endod J 2004 Sep;37(9):607-612.
  24. Passive ultrasonic irrigation of the root canal: a review of the literature. Int Endod J 2007 Jun;40(6):415-426.
  25. Final rinse optimization: influence of different agitation protocols. J Endod 2010 Feb;36(2):282-285.
  26. Effect of EDTA with and without surfactants or ultrasonics on removal of smear layer. J Endod 2007 Apr;33(4):472-475.
  27. The influence of filling technique on depth of tubule penetration by root canal sealer: a study using light microscopy and digital image processing. Aust Endod J 2004 Apr;30(1):23-28.
  28. Antimicrobial activity and flow rate of newer and established root canal sealers. J Endod 2000 May;26(5):274-277.
  29. Sealer penetration and adaptation in the dentinal tubules: A scanning electron microscopic study. J Endod 2011 Nov;37(11):1576-1579.
  30. Effect of tubule orientation in the cavity wall on the seal of dental filling materials: an in vitro study. Int Endod J 1998 Sep;31(5):326-332.
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.